Generator
CLuc-NLuc

Part:BBa_K2845006:Design

Designed by: Antonio Martínez, Jan Krüsemann, Eline Bijman   Group: iGEM18_ETH_Zurich   (2018-10-10)


Split EPIC luciferase in polycistronic mRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 495
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 199


Design Notes

C-terminus and N-terminus of split EPIC Luciferase (BBa_K325101) on a polycistronic mRNA under the control of a strong Anderson promoter and a medium RBS. Designed for testing the background luminescence through random self-assembly of the two split luciferase halves.